On a standard reticulocyte preparation with new methylene blue, 100 cells are counted containing blue-stained granulofilamentous material. The red blood count is 3.22 ´ 1012/L, and the hematocrit is 30%. Calculate the absolute reticulocyte count
a. 0.32 x 1012/L
b. 3.2 x 1012/L
c. 32.2 x 1012/L
d. 0.99 x 1012/L
ANS: A
The absolute reticulocyte count is = relative % reticulocytes ´ the red count. In this example, the absolute reticulocyte count = (0.10) x 3.22 x 1012/L = 0.32 x 1012/L.
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