When the ribosome reaches a stop codon on the mRNA, no corresponding tRNA enters the A site. If the translation reaction were to be experimentally stopped at this point, which of the following would you be able to isolate?
A. an assembled ribosome with a separated polypeptide
B. separated ribosomal subunits, a polypeptide, and free tRNA
C. an assembled ribosome with a polypeptide attached to the tRNA in the P site
D. a cell with fewer ribosomes
E. separated ribosomal subunits with a polypeptide attached to the tRNA
Ans: C. an assembled ribosome with a polypeptide attached to the tRNA in the P site
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a. negative change in kinetic energy b. positive change in enthalpy c. negative change in entropy d. DG = 0 e. negative change in free energy
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a. after three turns, during the Fixation stage b. after six turns, during the Regeneration stage c. after six turns, during the Reduction stage d. after eight turns, during the Fixation stage
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a. 8 b. 15 c. 23 d. 30 e. 38