A mutation in E. coli results in the loss of both restriction endonucleases and modification enzymes. Would you expect any difference in the frequency of gene transfer via transduction FROM Salmonella INTO this E. coli strain?
A. No-since the Salmonella strain is normal, the rate of production of transducing virus particles would still be the same, resulting in the same frequency of gene transfer.
B.
Yes-the loss of the restriction endonucleases would leave the recipient E. coli unable to break down "invading"' viral DNA from the transducing phage. This would lead to higher rates of successful transduction.
C.
Yes-the loss of the modification enzymes would leave the recipient E. coli unable to tag its own DNA as "self," leaving the viral DNA untagged and recognizable as "foreign," and targeted for destruction. This would lead to higher rates of successful transduction.
D. No-transduction efficiency isn't affected by either restriction endonucleases or modification enzymes, so there'd be no effect on the overall rate.
E.
Yes-the loss of the restriction endonucleases would leave the recipient E. coli unable to break down "invading" viral DNA from the transducing phage, AND the loss of the modification enzymes would leave the recipient E. coli unable to tag its own DNA as "self," leaving the viral DNA untagged and recognizable as "foreign," and targeted for destruction. Together, thesewould lead to higher rates of successful transduction.
E
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