Sudan IV, a dye that binds to nonpolar molecules, is used to detect the presence of macromolecules in food. Sudan IV would test positive in the presence of what macromolecule?
A. Starch
B. DNA
C. Hemoglobin
D. Chitin
E. Fats
Clarify Question
· What is the key concept addressed by the question?
· What type of thinking is required?
· What key words does the question contain and what do they mean?
Gather Content
· What do you know about nonpolar molecules? How does it relate to the question?
Consider Possibilities
· What other information is related to the question? Which information is most useful?
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
E. Fats
Clarify Question
· What is the key concept addressed by the question?
o The question asks about nonpolar molecules.
· What type of thinking is required?
o You are being asked to apply your knowledge of the polarity of different macromolecules to predict which would bind to a dye.
· What key words does the question contain and what do they mean?
o Sudan IV – a dye that binds to nonpolar molecules and turns those samples a color.
Gather Content
· What do you know about nonpolar molecules? How does it relate to the question?
o Nonpolar molecules are hydrophobic and do not dissolve in water. Lipids and fats are common nonpolar biological molecules.
Consider Possibilities
· What other information is related to the question? Which information is most useful?
o Hemoglobin is a protein found in blood, which is a water based solution, so it is a hydrophilic and polar molecule.
o Chitin and starch are complex carbohydrates with lots of hydroxyl groups, they are hydrophilic and polar molecules.
o DNA is highly charged and also a polar and hydrophilic molecule.
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
o Fats are nonpolar hydrophobic molecules, so they will bind to Sudan IV and turn any foods containing fats a color.
Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
o This question asked you to apply the properties of a macromolecule to determine if it would bind to a hydrophobic dye. If you got the correct answer, great job! If you got an incorrect answer, where did the process break down? Did you recall what nonpolar meant? Could you remember which biological molecules were nonpolar and which were polar?
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