Select the experiments that can be characterized as reverse genetics: (check all that apply)
_____ Generation and analysis of a knockout mouse
_____ Random mutagenesis of an organism, then selecting mutants by phenotype
_____ RNA interference of a gene in C. elegans
_____ Using reverse transcriptase to create a cDNA library
__X__ Generation and analysis of a knockout mouse
_____ Random mutagenesis of an organism, then selecting mutants by phenotype
__X__ RNA interference of a gene in C. elegans
_____ Using reverse transcriptase to create a cDNA library
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A drug binds to the active site of an enzyme. If it is bound to the active site of the enzyme, it prevents substrate binding. This drug would be considered a
A. noncompetitive inhibitor. B. allosteric inhibitor. C. allosteric activator. D. competitive inhibitor.
Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on
the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ____.
a. recombinant; deleted from the plasmid b. nonrecombinant; intact c. recombinant; intact d. nonrecombinant; disrupted by the inserted DNA fragment e. recombinant; disrupted by the inserted DNA fragment
Isotopes are commonly used in medicine to determine if organs or organ systems are functioning properly. Physicians use radioactive iodine rather than elemental iodine to determine if the thyroid gland is functioning properly because:
1.Isotopes of iodine are radioactive, so they can be detected by imagery, and they act the same as elemental iodine. 2.Elemental iodine cannot be used by the human thyroid gland. 3.The isotope is more efficient than elemental iodine and would be processed by the thyroid gland at a higher rate. 4.Elemental iodine is in short supply.
Which of the following helps in chromosomal movement during cell division?
a. chloroplasts b. microtubule c. microfilaments d. peroxisomes e. intermediate filaments