Explain how the technique of exon trapping works

What will be an ideal response?


Ans: See Figure 5.8. Vectors that carry inserted genomic fragments containing novel exons flanked by partial introns allow splicing of the 5? splice site of the vector intron with the 3? splice site adjacent to the exon and splicing of the 3? splice site of the vector intron with the 5? splice site adjacent to the exon. This allows a correctly spliced mRNA to reach the cytoplasm and yields a spliced RNA fragment that is longer than the size of the initial spliced RNA fragment.

Biology & Microbiology

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he logistic equation:a

models a J-shaped growth curve. b. takes into account the carrying capacity of the environment. c. predicts continued exponential growth. d. would characterize the uncontrolled growth of a population. e. models a K-shaped growth curve.

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Which of the following choices CORRECTLY ranks by the routes of water loss from largest source of water loss to smallest source of water loss, by their average volume?

A. urine, skin, feces, lungs B. urine, skin, lungs, feces, C. urine, feces, skin, lungs D. skin, urine, lungs, feces

Biology & Microbiology

Which of the following cells is a component of the body's first line defense?

A) monocyte B) goblet cell C) NK cell D) neutrophil E) microglial cells

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Which of the following is the target for aldosterone?

a. proximal tubules b. loop of Henle c. distal tubules d. renin e. angiotensin II

Biology & Microbiology