In muscular dystrophy, muscle cells lack a protein called dystrophin, which leads to weakening of the muscles. As a biomedical researcher, your goal is to create a gene therapy approach to treating muscular dystrophy. You have a sequence of DNA that
contains the dystrophin gene and a virus that infects human cells. You want to combine the dystrophin gene with the viral DNA so that the virus can infect and insert the gene into the muscle cells as a way to treat the disease. Explain how you would get the dystrophin gene into the viral DNA.
What will be an ideal response?
The gene would be cut out of human DNA using restriction enzymes, and the viral DNA would be cut with the same restriction enzyme. This creates complementary sticky ends on both sources of DNA. The dystrophin DNA is then mixed with the viral DNA to create recombinant viral DNA.
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Crossing over is one of the most important events in
meiosis because a. it produces new combinations of alleles on chromosomes. b. homologous chromosomes must be separated into different daughter cells. c. the number of chromosomes allotted to each daughter cell must be halved. d. homologous chromatids must be separated into different daughter cells. e. all of these reasons are true.
Tight junctions contain
A. occludins and connexins. B. cadherins and claudins. C. claudins and integrins. D. cadherins and integrins. E. occludins and claudins.
The placement of an organism into a domain is made on the basis of
A) G + C content. B) cell ultrastructure. C) ribosomal RNA analysis. D) serological tests. E) Gram-stain reactions.
In the early 1900s, cities such as Philadelphia reduced the incidence of typhoid fever by
A) isolating human carriers. B) using tertiary water treatment systems. C) filtering municipal drinking water through sand-bed filters. D) requiring residents to boil drinking water. E) mass vaccination of residents.