You are a technician in a neuroanatomy lab, and it's your first day of training to learn a technique of labeling neurons. Your boss tells you that you will fill individual motor neurons, by injecting horseradish peroxidase (HRP) conjugated to tetanus toxin, into a skeletal muscle. How could this possibly label the neurons?
What will be an ideal response?
The process of labeling neurons by HRP was introduced in Chapter 9; this enzyme is taken up by axon terminals and transported retrogradely, filling most or perhaps all dendrites, as well as the axon and soma. When the substrate of the enzyme is added to tissue slices, a dark reaction product is formed, and this is how the details of the cell are visualized. Toxins such as tetanus and cholera toxin, which are also taken up and transported by axon terminals, enhance the uptake of HRP, if they are attached (conjugated) to the HRP.
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Why is the contraction strength of smooth muscle relatively independent of its resting length?
A. It is involuntary muscle. B. It gets nearly all of its Ca2+ from the extracellular fluid. C. It does not have intercalated discs. D. It is regulated by a length-tension relationship. E. It does not have sarcomeres.
Which of the following statements regarding vision is FALSE?
A. Cataract is an increase in opacity (clouding) of the lens. B. Presbyopia is a condition in which the lens cannot accommodate adequately for near vision. C. The image of an object that is focused on the retina is upside down relative to the object's actual position in space. D. Myopia is a condition in which the lens focuses light from distant objects behind the retina. E. The cornea refracts light rays more strongly than the lens.
Where is the suprachiasmatic nucleus (SCN) located?
a. thyroid b. adrenal medulla c. adrenal cortex d. thalamus e. hypothalamus
RNA that has the function of bringing amino acids to the ribosome during translation is called ________ RNA.
A. messenger B. transfer C. ribosomal D. nuclear