Which of the following would render an autoclaved load unsterile? 

A. Steam temperature of 212°F or 100°C
B. Wrapping the instruments in muslin
C. Air trapped inside the chamber because of blocked discharge lines
D. Placing hinged instruments in the open position
E. Refilling the water reservoir before operating


C. Air trapped inside the chamber because of blocked discharge lines
You should check the discharge lines before operating the autoclave, because blocked lines result in air being trapped inside the chamber, which makes the load unsterile.

Health Professions

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