There are three types of sequencing techniques described in the text: Sanger (dideoxy) sequencing, whole-genome

shotgun sequencing, and Illumina/Solexa sequencing. Which one(s) would be best to use for sequencing a single gene
versus an entire genome and why?



What will be an ideal response?


ANSWER: Single genes can be sequenced using the Sanger method since this technique, although
automated, can also be pared down to focus on a single gene or DNA fragment. All three
methods can be used for whole-genome sequencing. The Illumina/Solexa is used to sequence
whole genomes in an automated way, and would never be used to sequence a single gene or
fragment. Whole-genome shotgun sequencing is not really a sequencing technique, per se,
but is rather an approach to combining the data from sequencing genomic fragments after
using either the Sanger method or the Illumina/Solexa method to acquire the sequence data.

Biology & Microbiology

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