The Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard (M38-A2) describes the guidelines for antifungal susceptibility test-ing for molds. The suspension must be standardized with a spectrophotometer. Susceptibility testing is determined by which method?
a. Disk diffusion
b. Microdilution
c. Broth dilution
d. Immunodiffusion
B
M38-A2 is an approved microdilution method for antifungal susceptibility testing for molds; it also suggests using the Roswell Park Memorial Institute (RPMI)-1640 medium with 4-morpholinepropanesulfonic (MOPS) acid in yeast testing. The suspension must be standardized with a spectrophotometer. The amount of growth in each well should be compared with the growth in the control well.
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