Indicate the order of the point mutations in the rII region.

Shown below are the maps of a series of rII- deletion strains (1–5). The deleted region is indicated as (......) and the intact region as ______. Note that strain 5 carries two different deletions.



1 ___________(...........)_______________





2 _________________(...........)_________





3 (.....................)_____________________





4 ________________________(................)





5 _____(..........)________________(.........)



rII- phage strains A-E have point mutations in the rII region. E.coli K(?) cells are coinfected with one phage that has a deletion and one phage that has a point mutation. The presence of wild-type progeny phage is assessed by the presence (+) or absence (o) of plaques.







A) CADBE

B) DEBAC

C) BADCE

D) ABDEC

E) CEADB



Additional question (answer with TRUE or FALSE) :



The test described here depends on recombination.


A) CADBE

Additional answer : FALSE

Biology & Microbiology

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