In 2D chromatography analysis, each of the spots on the chromatography paper represent different proteins. In order to
correctly identify the proteins and interpret the data, which experimental control must be included?
a. using cellular carbohydrate extract that was not labeled with radioactivity to determine the effect of radioactive
labeling on the study
b. using purified proteins of interest in individual trials to identify each protein's profile on the chromatography
paper
c. using water in place of the cellular carbohydrate extract to compare the profile of the proteins to a completely
pure sample
d. using water in place of the solvent to determine the effect of the solvent on the movement of the proteins on
the chromatography paper
e. removing excess proteins from the cellular carbohydrate extract before chromatography
ANSWER: b
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