To generate a knockin mouse, a scientist introduces to ES cells a construct with a mutant exon and within an adjacent intron, loxP sites flanking a neomycin resistance gene. What event will occur next?
A) Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.
B) Recombination between the loxP sites.
C) Transplantation of cells into host blastocyst.
D) Crossing of heterozygous mice to a Cre-expressing strain.
A) Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.
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PCR is the name of the reaction used to amplify specific sequences of DNA
A. true B. false
You run a PCR cycle for five cycles. Theoretically, how many copies of your sequence would you now have?
A. 8 B. 12 C. 16 D. 24 E. 32
When threshold potential is reached, which of the following occurs?
a. The activation gates of Na+ channels open. b. The inactivation gates of Na+ channels open. c. The activation gates of K+ channels open. d. The inactivation gates of Na+ channels open. e. Na+/K+ active transport pumps are activated.
Insulin affects the
a. secretion of saliva. b. storage of proteins. c. secretion of pancreatic juices. d. metabolism of sugar. e. utilization of fat reserves