A polymerization reaction is made irreversible by:

A. hybridization of a free nucleotide to a template
B. breaking of the bond between the 2' carbon and the hydroxyl group
C. creation of a phosphodiester bond
D. hydrolysis of a pyrophosphate group into two inorganic phosphates
E. entry of a free nucleotide into an RNA polymerase


Answer: D

Biology & Microbiology

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