In an in vitro translation assay radioactive amino acids can be incorporated into proteins and the proteins separated from the unincorporated amino acids
Given the following data what are the conclusions that you would come to?
RNA added to reaction Amount of radioactivity in protein in counts per minute
Insulin mRNA alone 10,000
Insulin mRNA with antisense insulin mRNA 3,000
Insulin mRNA with antisense fibronectin RNA 11,000
No mRNA 150
Insulin mRNA with double stranded insulin RNA 500
A. Double stranded RNA is more efficient at inhibiting translation than antisense RNA
B. Antisense RNA is more efficient at inhibiting translation than double stranded RNA
C. No conclusion about the efficiency of translation inhibiton can be made as the positive and negative controls did not perform appropriately
D. The experiment did not work since the antisense fibronectin did not have an effect on amino acid incorporation.
Blooms Level:
A
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