Which situation(s) might warrant the removal of a histidine tag when purifying a recombinant protein?

A. Histidine residues inhibit protein folding, thereby decreasing the functionality of the protein.
B. The expression vector used for inserting the gene of interest already contains histidine-encoding sequences.
C. Histidine residues bind preferentially to nickel resins on the column during purification, and are not washed away.
D. All of the choices are correct.


Answer: A

Biology & Microbiology

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