You want to insert your favorite gene, which has been digested with Ncol endonuclease on both ends, into a vector. Ncol recognizes the DNA sequence C*CATGG. The vector you want to use contains many endonuclease recognition sites, but does not include an Ncol site. Which restriction endonuclease would be the most suitable replacement to digest the vector?
A. Sphl - G*CATGC
B. Kpnl - GGTAC*C
C. Sunl - C*GTACG
D. CViAll - C*ATG
Answer: A
You might also like to view...
Which of the following choices would most likely promote random distribution?
A) territorial species B) species that secrete chemicals to attract or inhibit other individuals C) flocking and schooling behaviors D) spacing during the breeding season E) homogeneous chemical and physical factors in the environment
The process of apoptosis can remove structures such as mammary tissue in male mammals
a. True b. False
Which of the following would be the first step in the biosynthesis of a virus with reverse transcriptase?
a. A complementary strand of RNA must be synthesized. b. Double-stranded RNA must be synthesized. c. A complementary strand of DNA must be synthesized from an RNA template. d. A complementary strand of DNA must be synthesized from a DNA template. e. none of the above
Which of the following statements about hormones is NOT true?
A. Hormones usually affect a target organ. B. Hormones may be classified as peptides or steroids. C. Hormones are secreted into the bloodstream. D. Cells that react to a hormone have specific receptors for that hormone. E. Hormones are released from exocrine glands.