What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the ´40 objective?
a. Condenser not lowered enough
b. Hemocytometer not cleaned properly
c. Oil on the 40´ lens
d. Cells not allowed to settle
ANS: C
Oil should never be allowed to touch any lens that is not an oil immersion lens. If it mistakenly does get on the 40´ lens, then the object will appear fuzzy and unclear. The condenser should be in the highest position for best visualization. If a hemocytometer is dirty, then it will make it difficult to distinguish cells from debris. If cells are not allowed to settle, then they will not all be in the same plane of focus and some may be missed for counting purposes.
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