How do the techniques of PCR and RT-PCR differ?
What will be an ideal response?
Ans: The polymerase chain reaction (PCR) is a technique to "amplify" a fragment of DNA to numbers suitable for analysis. RT-PCR adds a step prior to this, reverse transcribing RNAs to DNA before using PCR to amplify the DNA. RT-PCR allows, for example, the expressed genes (lacking introns) of a cell sample to be amplified.
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Xeroderma pigmentosum (XP) is a rare autosomal recessive disorder. Patients with XP exhibit a cellular hypersensitivity to ultraviolet (UV) radiation, a high incidence of skin cancer and premature aging. Based on these clinical characteristics, what is the most likely cause for this disease?
A. defects in DNA repair B. defects in DNA replication C. lack of telomerase activity D. shortened telomeres Clarify Question · What is the key concept addressed by the question? · What type of thinking is required? · What key words does the question contain? Gather Content · What do you already know about DNA replication and repair? Consider Possibilities · What other information is related to the question? Which information is most useful? Choose Answer · Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer? Reflect on Process · Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
Arrange the following terms from most inclusive to least inclusive
1. embryophytes 2. green plants 3. seedless vascular plants 4. ferns 5. tracheophytes A) 1, 2, 5, 3, 4 B) 2, 1, 5, 3, 4 C) 2, 5, 1, 3, 4 D) 1, 4, 2, 5, 3
Which of the following statements is true of eukaryotic DNA?
a. A promoter is part of an intron b. An intron is part of a promoter c. Introns are sequences of DNA d. Exons are edited out of pre-mRNA
Suppose a population splits into two descendant populations prior to any evolution of dark coloration, then splits again after the evolution of dark coloration. What is the scientific term used for this situation?
a. a homoplasy c. a synapomorphy b. an analogy d. a symplesiomorphy