Your colleagues are attempting to study the citric acid cycle in the lab. To do so, they have dissolved oxaloacetate in water, then stirred in sufficient quantities of every required enzyme, coenzyme, and oxidizing agent, before adding ADP and acetyl CoA. After waiting a sufficient period of time, they test the solution for ATP and are disappointed to find significantly less ATP than expected. What is the flaw in their experiment?
a. Pyruvate dehydrogenase is not water soluble and is unable to convert pyruvate into acetyl CoA outside mitochondria.
b. Stirring the solution will introduce oxygen molecules, which will bond to ADP, preventing further phosphorylation.
c. Without subsequent pathways to oxidize NADH and FADH2, no NAD+ and FAD are available to continue the cycle.
d. ADP molecules spontaneously dephosphorylate in the presence of acetyl CoA outside of mitochondria.
c. Without subsequent pathways to oxidize NADH and FADH2, no NAD+ and FAD are available to continue the cycle.
You might also like to view...
The ____ in mammals allows water to exit but does not allow ions or urea to exit
a. distal convoluted tubule b. Bowman's capsule c. descending segment of the loop of Henle d. proximal convoluted tubule e. ascending segment of the loop of Henle
What are the major trophic groups of terrestrial microbes and where in terrestrial ecosystems are they found?
What will be an ideal response?
Which of the following nucleotide changes leads to a transition mutation?
A) Adenine to guanine B) Adenine to cytosine C) Guanine to cytosine D) Thymine to guanine E) Guanine to thymine
What is true of the corpus luteum?
A. The major hormone secreted from the corpus luteum is LH. B. Secretions of the corpus luteum stimulate LH and FSH secretion. C. Secretions of the corpus luteum induce maturation of primary oocytes. D. The major hormone secreted from the corpus luteum is estradiol. E. Secretions of the corpus luteum prepare the uterus for implantation of the embryo.