What type of procedure is appropriate to isolate revertants from a population of threonine auxotrophic cells?
A. Grow the auxotrophic cells on and agar plate with threonine; any colonies that form are presumed revertant.
B. Grow the auxotrophic cells on an agar plate without threonine; any colonies that form are presumed revertant.
C. Threonine auxotrophic revertants could not be isolated on any type of media.
D. Grow the auxotrophic cells in broth with threonine; if the broth becomes cloudy, revertants are presumed present.
Answer: B
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