Using specific examples, when is it appropriate to use CARD-FISH rather than the traditional FISH method and vice-versa?

What will be an ideal response?


Answer: CARD-FISH is especially useful when the target nucleotide sequence is at a relatively low concentration such that the fluorescent signal using traditional FISH is not strong enough to detect. While 16S rRNA-targeted probes might work in observing cells with a high copy number, other low copy number target sequences such as mRNAs (e.g., catabolic pathway transcripts) usually require CARD-FISH to detect the target sequence.

Biology & Microbiology

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Which of the following represents an activity

within a population?

a. a fox consuming a rabbit b. the absorption of nitrogen by bacteria and converting it to a form useful to plants c. a peacock spreading and shaking his feathers to attract a female d. moss growing on the north side of a large pine tree e. a virus causing rabies in a dog

Biology & Microbiology

How many levels of consumption are depicted in the

figure? a. 1 b. 2 c. 3 d. 4 e. 5

Biology & Microbiology

How does the location of DNA in animal cells compare to DNA's location in plant cells?

What will be an ideal response?

Biology & Microbiology

Which of the following supposedly speeds the removal of lactic acid from contracting muscles?

What will be an ideal response?

Biology & Microbiology