Which methodology was used by researchers to identify the nuclear localization sequence?
a. infection of cells in culture with viruses containing sequential deletions of amino acids in a protein known to
localize to the nucleus, followed by microscopic determination of cellular localization
b. treatment of cells in culture with pharmacological agents that inhibit the nuclear pore complex, followed by
microscopic determination of cellular localization
c. transfection of cells in culture with mutant human proteins containing sequences believed to be required for
nuclear localization followed by microscopic determination of cellular localization
d. fluorescently labeling cells with known nuclear proteins and tracking changes in localization in the presence or
absence of nuclear pore complex proteins
e. treatment of cells in culture with pharmacological agents that binds to the nuclear localization sequence and
prevents nuclear transport, followed by microscopic determination of cellular localization
ANSWER: a
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The wing of a bat, the flipper of a whale, and the arm of a human have a similar structure and all can trace their lineage back to a common ancestor. This would be an example of which of the following?
a. a homologous structure b. a vestigial structure c. an analogous structure d. none of the above
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A(n) ________ is composed of one sugar (deoxyribose or ribose), one phosphate, and one nitrogenous base
Fill in the blank(s) with correct word