In the identification of mycobacterial isolates, the Tween 80 test involves:
a. an enzyme that is able to produce Tween 80 from certain ingredients found in the medium.
b. a lipase that is able to hydrolyze polyox-yethylene sorbitan monooleate into oleic acid and polyoxyethylated sorbitol.
c. the metabolism of niacin to nicotinic acid by enzymatic action.
d. testing the isolate for susceptibility to Tween 80.
B
The commonly nonpathogenic, slow-growing scotochromogens and nonphotochromogens pro-duce a lipase that is able to hydrolyze Tween 80 (the detergent polyoxyethylene sorbitan mo-nooleate) into oleic acid and polyoxyethylated sorbitol, whereas pathogenic species do not. This test is useful in differentiating these species.
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