What type of microscope would you use to visualize the internal structures of a chloroplast?

Support your conclusion with evidence based on the size of the structures you want to see and the resolution and magnification power of different types of microscopes.
What will be an ideal response?


Answer: Transmission electron microscopy would be necessary to visualize the internal structures of a chloroplast. Chloroplasts are less than 5 μm in diameter and the internal membranes are only 10 nm thick. Light microscopes only have a resolution of 200 nm, thus any structure less than 200 nm will not be visible. Individual chloroplasts could be seen with a light microscope, but not the structures inside. Scanning electron microscopy can only see external features because electrons cannot penetrate the cell, thus the cell must be sectioned and prepared for transmission electron microscopy to see the inside of the chloroplasts.

Biology & Microbiology

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You are studying a biochemical pathway. Preliminary results suggest that this pathway is subject to end-product repression. This pathway is therefore most likely to be a(n) ________ pathway.

A) anabolic B) catabolic C) constitutive D) induced E) unregulated

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DNA replication is considered semiconservative because

A. it uses one old strand as a template for the synthesis of a new strand. B. it never replicates in the 5' to 3' prime direction. C. it always replicates in the 3' to 5' prime direction. D. it will create three new identical strands when finished.

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How can the same first messenger evoke different responses in different target cells?

a) it depends on the tissue cAMP binds to b) it depends on the specific proteins phosphorylated by PKA c) none of the choices is correct d) it depends on the ligand cAMP binds to

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All surviving hominins belong to the species

A) Homo habilis. B) Homo sapiens. C) Australopithecus robustus. D) Homo robustus. E) Homo erectus.

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