Describe how data are collected and displayed in flow cytometry
What will be an ideal response
In flow cytometry, multiple measurements are taken of each cell (side scatter, forward scatter, fluorescence of
antibody tags). Data from each cell are placed in categories (channels) based on the intensity of the signal. The data
can be manipulated to view one parameter at a time or multiple parameters at one time. If one measurement is
viewed, the typical display is a frequency distribution or histogram showing the number of cells possessing each
category of signal intensity. If more than one measurement is to be viewed simultaneously, the display can be a two
parameter dot plot or scatter plot. Typically, the operator first selects a particular cell population to analyze. Using
forward/side scatter characteristics, the cell population (monocytes, granulocytes, lymphocytes) is selected in a
process called gating, and the histogram/plot display is restricted only to cells in the gate.
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