You have isolated DNA for PCR and accidentally added topoisomerase instead of polymerase to your sample. What would you expect if you ran this DNA sample on a gel?
A) The DNA would show increased supercoiling and have a lower electrophoretic mobility.
B) The DNA would show decreased supercoiling and have a higher electrophoretic mobility.
C) The DNA would show increased supercoiling and have a higher electrophoretic mobility.
D) The DNA would show decreased supercoiling and have a lower electrophoretic mobility.
E) The DNA would show decreased supercoiling, but electrophoretic mobility will be unaffected.
D
You might also like to view...
Coliforms
A. are an informal grouping of enterics. B. ferment lactose. C. include E. coli. D. are used as indicators of fecal contamination. E. All of the choices are correct.
Which one of the following is NOT transmitted by Aedes mosquitoes?
a) Malaria b) Yellow fever virus c) Dengue fever virus d) Chikungunya virus
Which neurotransmitter may function to regulate emotions and is involved in pathways that control complex movements?
A) synapse B) serotonin C) norepinephrine D) dopamine
All the following are correct about the polio vaccines, except
A. Dr. Jonas Salk developed the IPV vaccine in 1954. B. the inactivated polio vaccine (IPV) can occasionally revert to a neurovirulent strain. C. the virus is transmitted through the fecal-oral route. D. most infections do not result in paralysis. E. treatment for polio involves mainly alleviating pain and suffering.