In an effort to clarify and standardize microbial taxonomy, the International Committee on Systematics of Prokaryotes (ICSP) recommends four criteria to meet a "gold standard" for species assignment. Which of the following is not part of the criteria?
A. Less than 3% divergence in rRNA gene sequence
B. Whole genome similarity (as determined by DDH) of at least 70%
C. Melting temperature of the DNA (reflection of the G+C content) within 58°C
D. Bioinformatics ecotype analysis showing similar environmental niches
E. Phenotypic similarity to others microorganisms in the group
Answer: D
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