Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide. Why are ddNTPs integral to the Sanger sequencing method?

A) They have a 2? hydroxyl that allows for extension of the polynucleotide.
B) They do not have a 2? hydroxyl, which does not allow the extension of the polynucleotide.
C) They do not have a 3? hydroxyl, which does not allow the extension of the polynucleotide.
D) They have a 3? hydroxyl that allows for extension of the polynucleotide.


Answer: C

Biology & Microbiology

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