You are working in a cancer lab and are interested in identifying some genes that are important in the onset of lung cancer. You have several genes you think may be involved, so you "print" spots of the DNA sequences of interest on a microarray slide. After doing this, you isolate mRNA from both normal lung cells and cancerous lung cells. Before you use these mRNAs to probe the microarray slide, you need to

A. make cDNA and amplify the cDNA using PCR.
B. amplify the mRNA using PCR.
C. clone each mRNA into a BAC vector.
D. add reverse transcriptase and fluorescent nucleotides to the mRNA to make cDNA.
E. sequence the mRNA.


D. add reverse transcriptase and fluorescent nucleotides to the mRNA to make cDNA.

Biology & Microbiology

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