The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming
This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. Why would such a heat-stable polymerase be beneficial in PCR?
A) Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq polymerase.
B) Each cycle includes a "hot" saturation phase (95°C), which allows the primers to anneal to the target DNA.
C) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of the template DNA together.
D) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the culture.
E) More than one of the above are correct.
C
You might also like to view...
If a toxin blocks the receptors for the neurotransmitter on the postsynaptic muscle cell,
____________________.
a. paralysis will occur b. the person will have a seizure c. the action potential in the presynaptic cell fails d. calcium, which can function as a neurotransmitter, will be released
The level of organization where factors such as
sunlight, rainfall, and temperature enter the picture is the a. organ system. b. ecosystem. c. biosphere. d. molecule. e. community.
Independent assortment means that _____
A. monohybrid crosses will show segregation and independent assortment B. the segregation of one gene pair depends on the segregation of another gene pair C. the gametes produced must be heterozygous in all cases D. the segregation of one gene pair occurs as if no other gene pair was present E. the phenotypic ratio in F2 will be the same for dihybrid and monohybrid crosses
What is the process by which cells of a malignant tumor can break away and start new cancers at distant locations?
What will be an ideal response?