Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduced into a strain of E. coli. To better the odds of getting expression of the eukaryotic gene in this system, you need to make certain that
A) the gene has a eukaryotic promoter for transcription.
B) the plasmid has a prokaryotic promoter for transcription.
C) the gene and the plasmid were cut with different restriction enzymes.
D) the gene product is toxic to the bacterial cell.
E) mRNA is used for ligation directly into the vector.
B) the plasmid has a prokaryotic promoter for transcription.
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Isopropyl alcohol wiped across a skin site can sterilize it.
Answer the following statement true (T) or false (F)
Cloning eukaryotic organisms involves the transfer of a ________ into a cell that lacks one to direct the development of a new organism
Fill in the blank with correct word.
A gene that affects, prevents, or masks the expression of a gene at another locus is a(n) ____ gene
a. recessive b. dominant c. epistatic d. codominant e. pleiotropic
Which of the following occurs when a reaction reaches equilibrium?
a. The rates of the forward and reverse reactions are equal. b. The chemical reactions cease. c. Entropy is no longer in the system. d. The concentration of reactants equals the concentration of products.