Explain the purpose of the following used in the standard hematologic cell-counting detection methods:
a. VCS flow cell
b. Photodetector
c. Sheath fluid
d. Direct current detection block
What will be an ideal response?
Answer:
VCS flow cell: to detect each WBC's volume, conductivity, and scatter for determination of the five part differential
Photodetector: to measure the absorbance of the unknown blood sample passing through it (used in spectrophotometric detection of Hgb)
Sheath fluid: to promote hydrodynamic focusing of cells passing through photodetector
Direct current detection block: to detect electrical resistance when cells pass through the aperture
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