A 1:20 dilution of blood is made for a white count, and the following number of cells counted in the four large corner mm2 of the hemocytometer: 12, 28, 18, and 15. What should be done next?
a. Report the white count as 3.7 ยด 109/L.
b. Clean the hemocytometer and recharge with a well-mixed dilution.
c. Increase the number of mm2 counted.
d. Compare this cell count to the one on the other side.
ANS: B
The distribution of cells in these four mm2 is unacceptable (exceeds 10 cells); this is not random distribution. Unacceptable distribution makes the cell count invalid. Ideally, another dilution should be made, and it should be well mixed before charging the chamber. Other possible reasons for poor distribution include uneven flow when charging the chamber, dirt or oil on the surface of the chamber or coverslip, and disturbing the coverslip while counting the cells.
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