A large region of the F plasmid (~33 kb) is called the transfer region (tra) and contains about 40 genes necessary for conjugation. The gene traA codes for the protein pilin that is polymerized to form a pilus, using a number of other tra genes
The pilus is a hollow cylinder whose units disassemble once contact is made in order to "reel in" the recipient cell. What other functions of the tra genes are required for transfer? A) DNA unwinding, and prevention of contact with F+ cells
B) enzymatic DNA replication
C) resistance to phage infection
D) integration of F genes into the recipient's chromosome
E) integration of F genes into the recipient's plasmid
A
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