You have identified a protein that you hypothesize is a sigma factor in E. coli that has not yet been characterized. You have purified the protein. What types of experiments could you perform to investigate whether or not the protein is a sigma factor?
What will be an ideal response?
Answer: There are many possible experiments that would all contribute to the verification of this factor as a potential sigma. These include, but are not limited to, EMSA showing the factor binds preferentially to a specific sequence and the use of deletion mutations lacking promoter sequences to determine what sequences the hypothetical sigma factor binds to.
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For water to be transported to the top of a tall tree, several forces must be at work. From the choices below, which combination of forces explains this sort movement?
a. turgor pressure and evaporation b. water heat capacity and pH c. cohesion and transpiration d. gravity and conductivity
The poor fishing off the coast of Peru
associated with El Niño is related to oceanic a. lack of typical oceanic upwellings. b. upwellings. c. thermal inversions. d. cyclonic activity. e. oxygen depletion
What are the two different phases of VMH syndrome?
What will be an ideal response?
Which statement is not true about T cell subclass differentiation?
A) T cell subclass differentiation is caused by the release of cytokines that is influenced by the antigen's nature and the amount present. B) Regardless of which T helper cell subclass develops during an immune response, the humoral response is not impacted. C) Particular combinations of cytokines will favor the development of some T cell subclasses over others. D) Each subclass will include both effector and memory cells. E) Which specific T helper cell subclass that develops may impact the progression and outcome of an infectious disease.