A lesion from an individual that works in a research laboratory is cultured and reveals a "fried egg" appearance with a dark center when grown on blood agar after 48 hours of incubation. The organism is nonmotile, indole, catalase, oxidase, and nitrate negative. Which of the following organism was isolated?
a. Streptobacillus sp.
b. Actinobacillus sp.
c. Aggregatibacter aphrophilus
d. Cardiobacterium sp.
A
Streptobacillus sp. growth occurs on blood agar, incubated in a very moist environment with 5% to 10% carbon dioxide (CO2), usually after 48 hours of incubation at 37°C. Colonies are embed-ded in the agar and may also have a "fried egg" appearance, with a dark center and a flattened, lacy edge. S. moniliformis does not produce indole and is nonmotile, catalase, oxidase, and ni-trate negative. Actinobacillus sp. and A. aphrophilus are nitrate positive, Cardiobacterium sp. are indole positive.
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