Sodium hydroxide (NaOH), Zephiran–trisodium phosphate, N-acetyl-L-cysteine–NaOH (NALC-NaOH), and oxalic acid are associated with:

a. methods of cultivating Mycobacterium in vitro.
b. digestion and decontamination of speci-mens for mycobacterial studies.
c. identification of clinically significant Mycobacterium.
d. antimicrobial susceptibility testing of mycobacterial species.


B
Commonly used decontamination agents are NaOH and NALC-NaOH. Oxalic acid is useful for treating specimens known to harbor gram-negative rods, particularly Pseudomonas and Proteus, which are extremely troublesome contaminants.

Biology & Microbiology

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