Describe a method used to visualize the location of a protein in a cell
What will be an ideal response?
One method is immunofluorescence, which uses an antibody to the protein of interest. This antibody or a second antibody that can bind to the first will be attached to a fluorochrome or to biotin. The resulting fluorescence can be detected with a microscope.
If the protein is not particularly antigenic, a fusion protein can be made with an epitope tag and an antibody to the epitope can be used. Another method involves fusing the protein to green fluorescent protein (GFP) which is autocatalytic (fluoresces on its own).
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The genetic material of prokaryotes is
a. RNA. b. linear. c. circular. d. single-stranded DNA. e. protected within a double-membrane structure.
What discovery by T. H. Morgan finally determined that genes were carried on chromosomes?
A. the finding of complete dominance B. discovery of the Rh blood group C. sex-linked inheritance of eye color in fruit flies D. heat-sensitivity of certain enzymes determining coat color
Acid-fast staining was performed on a sputum sample from a patient exhibiting clinical signs of Tuberculosis, red cells were observed on the slide. What can be determined from this slide?
A) The sample contained endospore forming bacteria B) The sample contained non-acid-fast cells, suggestive of members of the genus Mycobacterium C) The sample contained bacteria with mycolic acid in their cell walls, suggestive of members of the genus Mycobacterium
If you are modeling shopping decisions at the grocery store, and construct an outcome variable that is coded as 0 for no soup purchases, 1 for soup purchased of only one brand, and 2 for soup purchase of multiple brands, it would be appropriate to model this outcome as limited dependent variable because the:
A. outcome variable is ordinal. B. variable is dichotomous. C. range of possible values has consequential constraints. D. outcome variable is cardinal.