Outline a procedure for producing a useful new recombinant product
What will be an ideal response?
Many variations are possible. The answer should include (1 ) what organism is used as the source of DNA and how the desired DNA sequence is obtained, (2 ) the insertion of the DNA sequence into a plasmid, (3 ) the method of introduction of the plasmid into cells, and (4 ) how recombinant cells are identified. Since the question states a goal of a recombinant product, an expression vector should be specified. A variety of tools and techniques will be involved in a full answer.
Bloom's Taxonomy: Synthesis
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9, 8.10, 8.13, 8.14, 8.16
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A researcher is studying eukaryotic cells that shows an abnormal pattern of surface glycosylation. The researcher determines that these cells have normal levels of glycolipids and integral membrane proteins with O-linked glycosylation. The researcher discovers that there are no N-linked glycosylated proteins on the cell surface and unusually high levels of glycosylated in the ER membrane. What is a reasonable hypothesis to explain this observation?
A. The cell is defective in synthesizing of carbohydrates B. The cell is defective in assembling carbohydrate trees C. The cell has a defective oligosaccharide transferase D. The cell has a defect in its protein sorting pathway E. The cell has a defect in its ER localization signal
Rheumatoid arthritis is caused by
a. a deficiency of antibodies. b. inability to respond to antigenic stimulation. c. a virus. d. a self-attack on blood tissue. e. a self-attack on joints.
The first heart sound (lub) is produced by the closing of the __________ and __________ valves
a. aortic semilunar; pulmonary semilunar b. right atrioventricular; aortic semilunar c. left atrioventricular; pulmonary semilunar d. right atrioventricular; left atrioventricular e. mitral; left atrioventricular
Which of the following statements about regulation of thyroid hormones is true?
a) TSH positively feeds back on the hypothalamus causing TRH release b) T3 and T4 negatively feed back on the pituitary to reduce TSH release c) T3 and T4 negatively feed back on the pituitary to reduce TRH release d) TSH negatively feeds back on the hypothalamus to reduce TRH release e) Both b and d are true