When is it appropriate to use an artificial chromosome vector? Describe a specific example
What will be an ideal response?
Answer: Artificial chromosome vectors are generally best suited for carrying large inserts of DNA compared to other traditional plasmids. During DNA sequencing of an organism, several overlapping reads are necessary to develop a consensus sequence and therefore mandates a large number of different inserts to be sequenced. With the example of the human genome, an immense number of 1,000 bp fragments would need to be sequenced, all housed within an isolated bacterium containing the plasmid. Larger artificial chromosome decrease the complexity of genome sequencing projects by containing larger inserts (e.g., 800,000 bp fragments) so that fewer individual cells and fewer artificial chromosome vectors need to be sequenced.
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ATP hydrolysis directly powers the movement of _____
A) K+ out of cells B) Na+ out of cells C) Na+ into cells D) Ca++ into cells E) Cl- into cells
Natural selection operates directly on
(a) the genotype. (b) individual alleles. (c) the phenotype. (d) color only.
How is the net primary productivity of an ecosystem calculated?
What will be an ideal response?
The calcium that triggers muscle contraction is stored in the
A) motor neuron. B) plasma membrane of cells. C) endoplasmic reticulum. D) mitochondria.