The 3-day test that is useful for identifying the rapid-growers, M. fortuitum and M. chelonae, and that measures the rate by which this enzyme breaks down phenolphthalein disulfate into phe-nolphthalein and other salts is the ____________ test

a. niacin.
b. phenolphthalein.
c. arylsulfatase.
d. Tween 80.


C
The enzyme arylsulfatase is present in most mycobacteria. Test conditions can be varied to dif-ferentiate different forms of the enzyme. The rate by which this enzyme breaks down phenolph-thalein disulfate into phenolphthalein, which forms a red color in the presence of sodium bicar-bonate, and other salts helps differentiate certain strains of mycobacteria. The 3-day test is par-ticularly useful for identifying the potentially pathogenic rapid-growers, M. fortuitum and M. chelonae.

Biology & Microbiology

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