Why does the polymerase chain reaction require the samples to undergo numerous cycles, each
containing three different temperatures?
a. The initial cycles don't produce many copies of the original gene since the amplification is
exponential in nature.
b. The three temperatures are for different processes; the highest temperature separates the
double stranded DNA into single strands, the lower temperatures allow a target primer to
bind and a DNA polymerase to generate new sequences.
c. The enzyme used is from an organism that thrives in high temperatures, so high
temperatures must be used.
d. Non-target DNA is made during the first 10 cycles so further cycles are required to
specifically amplify the target DNA.
e. Once the enzyme starts working, you cannot stop the reaction until there are no more
nucleotides.
A
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