Selection of culture media for specific specimens includes a variety of media for all of the following reasons except:
a. a nutritive media is included to insure growth of all potential pathogens.
b. a selective media is often included to sup-press normal microbiota or other potential contaminating organisms.
c. as enrichment media is utilized to cultivate organisms that do not grow on routine media.
d. a back-up broth is included to provide in-oculum for additional testing.
D
Typically microbiology laboratories will include several media that enhance the growth and identification of the pathogens that are known to be present in each specific specimen or site of infection. Isolation media includes a general nutritional media to support growth of most organ-isms, a selective media to suppress normal microbiota or contaminating organisms, an enrichment media for fastidious organisms depending on the specimen and a back-up broth to enhance the growth of organisms that may be present in low numbers that do not grow on the agar plates. However, a back-up or enrichment broth must be plated to media to provide isolated colonies for further additional testing if no growth is identified on the primary media.
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