What is the recommended method for establishing accuracy of automated hematology analyzers?
a. Use at least three levels of controls for calibration.
b. Use whole blood calibration methodology.
c. Use commercially prepared whole blood calibrators.
d. Perform manual methods for comparison.
ANS: C
Whole blood calibrators are commercially available and are used for calibration of instruments. However, inherent problems exist with their use because these are stabilized and preserved cell suspensions that can deteriorate, and the calibration must be validated before the instrument is used for patient specimen analysis.
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