Another step in PCR requires small single-stranded DNA primers to anneal to a target sequence on denatured DNA. Two primers are used. Ideally these primers will have similar melting temperatures. The melting temperature is defined as the temperature at which 50% of the DNA is in the single-stranded form. Which of the following primers will have the highest melting temperature?

A. G G G A A A T T T C C C
B. A A A A G G C C T T T T
C. G G G G A A A T T T C C C C
D. A A A A G G G C C C T T T T


Answer: C. G G G G A A A T T T C C C C

Biology & Microbiology

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