Another step in PCR requires small single-stranded DNA primers to anneal to a target sequence on denatured DNA. Two primers are used. Ideally these primers will have similar melting temperatures. The melting temperature is defined as the temperature at which 50% of the DNA is in the single-stranded form. Which of the following primers will have the highest melting temperature?
A. G G G A A A T T T C C C
B. A A A A G G C C T T T T
C. G G G G A A A T T T C C C C
D. A A A A G G G C C C T T T T
Answer: C. G G G G A A A T T T C C C C
Biology & Microbiology
You might also like to view...
Genes live at specific locations along a chromosome called a _______.
a. habitat b. locus c. niche d. genetic zone
Biology & Microbiology
Which level of the taxonomic classification system is the least specific?
a. domain b. order c. genus d. species
Biology & Microbiology
Salt marsh communities predominate in temperate and subarctic areas
Indicate whether the statement is true or false
Biology & Microbiology
Viruses cause most human cancers
Indicate whether the statement is true or false
Biology & Microbiology