I tried to sequence a DNA fragment and ended up with the trace pattern shown in the figure above
What is the most likely cause of this result?
a. I forgot to add the ddCTPs to the sequencing reaction.
b. I forgot to add the normal dCTPs to the sequencing reaction.
c. I forgot to add DNA polymerase to the sequencing reaction mix.
d. The fluorescent tags for the ddGTPs are degraded.
e. The polyacrylamide gel didn't polymerize properly and is altering the migration patterns of
the DNA fragments.
A
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What will be an ideal response?
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The rate of diffusion through a membrane is dependent upon:
What will be an ideal response?