Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured (usually by soaking the gel in NaOH). Why is this step necessary?

What will be an ideal response?


to make the DNA single stranded so that the probe can anneal

Biology & Microbiology

You might also like to view...

Treponema pallidum is similar in shape to

A. Escherichia coli. B. Staphylococcus aureus. C. Leptospira interrogans. D. herpes virus.

Biology & Microbiology

Soils developing from sand and silt carried by the wind are referred to as

A) till. B) eolian. C) colluvium. D) fluvial.

Biology & Microbiology

Albinism is due to the inability to make ________

A) tyrosine B) melanin C) hair D) collagen

Biology & Microbiology

Which of our ancestors was "handy man"?

A. Homo habilis B. Homo erectus C. Australopithecus anamensis D. Australopithecus afarensis

Biology & Microbiology