Next-generation sequencing takes advantage of the concept of sequencing-by-synthesis. What is sequence-by-synthesis?
A) The use of firefly luciferase and pyrophosphate to report on nucleotide incorporation.
B) The use of a multiwell system and microfluidics to detect incorporated nucleotides at each base.
C) The use of a single polymerase in a nanopore that reports on a single molecule of synthesized DNA.
D) The use of PCR to incorporate ddNTPs, followed by electrophoresis.
Answer: B
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